Evaluation method for ion transport via nanopores: Toward a neural stimulation electrode using membrane protein

We intend to develop a new stimulation electrode that contains tubular membrane proteins. A “proteoelectrode” should be capable of controlling membrane potential through the pores formed by tubular membrane proteins. We have tested the formation of lipid bilayers and the insertion of alpha-hemolysin into them. An ion indicator method for measuring molecule transfer through alpha-hemolysin in the lipid bilayer was tested. Fluorescence imaging of the ion indicator showed the assembly of a lipid bilayer and the formation of alpha-hemolysin pores. This could be a simple and effective technique for evaluating the performance of a proteoelectrode.