Real-time monitoring on peptide synthesis by GMR biosensors

In this work, a real-time monitoring system has been developed. The micro-optic device applying the guided-mode resonance (GMR) effect has been designed and fabricated. By combining a simple and low cost microfluidic channel, the GMR measurement for detecting peptide synthesis reaction has been explored. It is able to determine whether or not the chemical reaction reacts and how far the chemical reaction arrived. In addition, the kinetics analysis for the peptide synthesis has also been discussed. It is successfully demonstrated that the GMR device is capable of monitoring the process of peptide synthesis in real time.