In silico analysis of nuclei in glioblastoma using large-scale microscopy images improves prediction of treatment response

In this paper, we present a complete and novel workflow for quantitative nuclear feature analysis of glioblas-toma using high-throughput whole-slide microscopy image processing as it relates to treatment response and patient survival. With a complete suite of computer algorithms, large numbers of micro-anatomical structures, in this case nuclei, are analyzed and represented efficiently from whole-slide digitized images with numerical features. With regard to endpoints of treatment response, the computerized analysis presents a better discrimination than traditional neuropathologic review. As a result, this analysis method shows potential to facilitate a better understanding of disease progression and patients' response to therapy for glioblastoma.