Optimization of conditions for schizophyllan production in submerged culture of schizophyllum commune

The effects of oleic acid, folic acid, citric acid, α-naphthalene acetic acid, L-glutamic acid, carboxymethylce-lluose, and Tween 80 at different concentrations on the schizophyllan production and mycelial growth in a submerged culture of S. commune CGMCC 5.113 at pH 6.5 and 26 °C were determined. The highest production of schizophyllan and biomass in submerged culture were 13.95 g/l and 27.56 g/l under the optimal conditions (oleic acid 0.1% (v/v), folic acid 1.5 mg/l, citric acid 0.2% (m/v), α-NAA 0.2 mg/l, L-glutamic acid 1.0 mg/l, CMC 0.8% (m/v), and Tween 80 5ml/l), respectively. The results of bioreactor fermentation also show that the optimal conditions enhanced schizophyllan production (13.68 g/l) and biomass (26.01 g/l) by S. commune in a 5–l fermentor.