Clinical applications of diffuse reflectance spectroscopy in normal and prediabetic subjects-A pilot study

A pilot study was conducted in 4 different regions on the foot sole of normal and prediabetic subjects to determine the ratios of oxy and deoxy hemoglobin bands using diffuse reflectance spectroscopy in visible range. The reflectance spectra of normal subjects showed a greater absorption dips at 542 nm. In conclusion the ratio of R542/R555, ratio of oxy and deoxy hemoglobin bands would be helpful in characterizing tissue oxygenation levels in normal and prediabetic subjects.