Study on NPR1 gene transformation by ovary injection pathway into watermelon (citrullus vulgaris)

Transformation of disease-resistant gene NPR1 to Watermelon was studied by ovary injection pathway after artificial pollination . Transgenic plants were obtained. Studies showed the injection of DNA solution at about 24 hours after pollination resulted in the highest transformation rate. PCR and southern blotting hybridization results showed that NPR1 gene were transformed into Watermelon.