Electrospun hemoglobin microbelts based biosensor for sensitive detection of hydrogen peroxide

Hemoglobin (Hb) microbelts based biosensor was developed by directly electrospinning Hb onto the glassy carbon electrode surface without using any other immobilization matrix, offering an excellent electrochemical sensing platform. UV-vis spectroscopy and FTIR spectroscopy were performed to demonstrate that Hb still kept its native structure in the as-electrospun microbelts. The electrocatalytic property of Hb microbelts modified electrode was investigated using H
₂
O
₂
as a model compound and the as-prepared biosensor showed fast response to H
₂
O
₂
, together with good dynamic response ranges, high sensitivity and excellent detection limits. These results indicated that Hb microbelts have great potential in the application of H
₂
O
₂
detection.