Low-voltage dielectrophoretic platform for Lab-on-chip biosensing applications

We propose in this paper a platform for bioparticles mixing and detection in Lab-on-chip (LoC) based device dedicated to neurotransmitters analysis. The proposed biosensing device is characterized by a low voltage dielectrophoretic separation using novel L-shape electrodes. In addition to the mixing architecture enabling reaction between different particles, liquid was also sampled using the same electrodes. Our design works with different low voltages depending on particle size. It is tested with microspheres in the range of micrometers with an applied voltage less than 5V. The system dimensions ar e reduced to the minimum size such as it needs only few picolitre liquid samples. In addition to have a better control and separation, it is crucial to design many in-channel electrodes with minimum dimensions. Thus the microchannel includes 32 L-shape electrodes. The width of each electrode is 10 μm separated by 10 μm. Consequently, the width of the microchannel is 650 μm. The number of electrodes was choosen based on the number of outputs available on the monitoring electrical circuit.