cDNA Cloning and Bioinformatic Analysis of Self-Incompatible S34-Allele from Chinese Pears

The full-length cDNA of a new S-RNase (
S

₃₄
-RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The
S

₃₄
-RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of
S

₃₄
-RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning of
S

₃₄
-RNase with other 39
S
-RNases of pears revealed the question about unifying naming system.