Electrical Detection of Biological Conjugation by AlGaN/GaN Heterostructure Field Effect Transistors

The article presents the electrical detection of streptavidin and monokin induced by interferon gamma proteins and hybridization process of single strand DNA by AlGaN/GaN HFETs. For protein conjugation detection, AlGaN gate surface was functionalized by oxidation, 3-aminopropyltriethoxysilane treatment, and biotinylation using NHS-sulfo-biotin. For streptavidin (STA) detection, at different ionic strengths, the effect of Debye length on detection sensitivity has been demonstrated. For DNA hybridization detection, 12-mer prime ssDNA with matched and mismatched sequences were used as probe and target molecules.