Study on a pyruvate oxidase biosensor based on ß-cyclodextrin included ferrocene as electron-transfer mediator

A pyruvic acid biosensor was constructed by the use of ferrocene which was included into the ß-cyclodextrin as electron transfer mediator as well as glutaraldehyde cross-linked membrane of egg white and ß-cyclodextrin as matrix of enzyme immobilization. Due to the excellent electron-transfer efficiency of ferrocene, the microenvironment from ß-cyclodextrin, and also the inclusion complexation between them, the biosensor has quite good properties. The linear detection limiting of this biosensor decreased to 5.68×10
^–7
mol/L with the excellent stability for 2.58% of relative standard deviation (RSD) during six individual detections.