Effects of EMP on Bone Formation of MC3T3-E1 Cells

The purpose of this study was to investigate the effects of electromagnetic pulse (EMP) on bone formation of MC3T3-E1 cells. Proliferation, ALP activity and mineralized nodule formation were examined in MC3T3-E1 cells after EMP exposure (field intensities: 0 kV/m, 50 kV/m, 400 kV/m, 400 pulses once a day for 7 days). After 50 kV/m EMP exposure, all the parameters of MC3T3-E1 cells were not changed significantly. However, the proliferation, ALP activity and mineralized nodule formation of MC3T3-E1 cells in 400 kV/m EMP exposure groups were decreased. In conclusion, the EMP we used was a possibly harmful factor for bone formation. It may suppress bone formation of MC3T3-E1 cells with higher electric field intensity.