Fast-scanning two-photon fluorescence imaging using a microelectromechanical systems (MEMS) two-dimensional scanning mirror

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce laser-scanning instrumentation based on a microelectromechanical systems (MEMS) scanner and describe two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.5 kHz. (180.2520) Fluorescence microscopy (180.5810) Scanning microscopy