Effect of EMP on lipid peroxidation of human gastric cancer cells MKN28

AIM
To discuss the effect of EMP on lipid peroxidation of human gastric cancer cells MKN28.
METHODS
MKN28 cells were divided into 5 groups (control group, 50, 100, 200, 400kV•
^-1
groups), 16 wells for each group. 12 hours after the cell-planting, each group were exposed by 0, 50, 100, 200, 400kV•m
^-1
respectively. 0, 6, 12, 24, 48h after EMP irradiation, the wells of each group were separated and detected by chemiluminescence analysator. The result was recorded in forms of value of first peak and 5s integral area. The data obtained from the experiment were analyzed by ANOVA.
RESULTS
Compared with control group, the chemoluminescence intensity (value of first peak and 5s integral area) of 50, 100kV•
^-1
irradiation groups (0, 6, 24h after irradiation) and 200, 400kV•
¹
irradiation groups (0, 6, 12, 24, 48h after irradiation) decreased significantly (p<0.01).
CONCLUSION
Certain EMP irradiation can significantly decrease the level of lipid peroxidation of human gastric cancer cells MKN28.