An evanescent fluorescence immunosensor based on K/sup +/ and Na/sup +/-exchanged patterned optical waveguides in glass

The authors describe, the successful fabrication of K/sup +/ and Na/sup +/-exchanged patterned waveguides and their application to wash-free immunoassays using evanescent excitation of fluorescence. Experimental results show that the specific binding of a fluorescently labelled antibody to its appropriate antigen could be resolved at the concentration level of 1 mu g/ml.<
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