Study of method and system for diagnosis of cancer using autofluorescence and Raman spectroscopy

Laser-induced fluorescence (LIF) and Raman spectra were measured from normal and tumorous human serum in an attempt to discover some values useful in discrimination between normal and tumorous cases. Red shift of fluorescence peak and decrease of fluorescence intensity were observed after samples radiated by laser. According to one thousand twenty-two samples' spectra, three parameters alpha, beta and Deltalambda are introduced to distinguish normal, benign and malignant from one another. The application of such parameters in clinical diagnosis was researched. The practical instrument of laser-induced serum fluorescence and resonance Raman spectra for cancer diagnosis or incipient cancer is designed, by combining laser spectroscopy, biomedical, photo-electron technology, controlling technology and computer technology. The instrument is intelligent for operating and diagnosing. The clinic application of this instrument has been carried out successfully in the diagnosing of stomach cancer and liver cancer (459 cases); the accuracy is about 85%. It develops a new technology in the field of cancer diagnosis