Nano-bio-molecular motors in microfabricated channels

We have investigated hierarchical capped channels that provide 3D microtubule confinement (Huang et al., 2004). We describe here a simplified polymethymethacrylate bonded (PMMA) 3D hierarchical channel process. This process provides shallow motor microchannels (4-6 μm wide and 1.5 μm deep) and deeper structures (~250 μm deep) for fluid input and output and allow simple electrode integration