Pixel-by-pixel classification of MFISH images

Multiplex Fluorescence In-Situ Hybridization (M-FISH) is a recently developed chromosome imaging method in which each chromosome is labelled with 5 fluors (dyes) and is also counterstained with DAPI. This paper proposes an automatic pixel by pixel classification algorithm for M-FISH images using a Bayes classifier. The M-FISH pixel classification was approached as a 25 class 6 feature pattern recognition problem. The classifier was trained and tested on non-overlapping data sets and an overall classification accuracy of 95% was obtained.