A disposable DNA sample preparation microfluidic chip for nucleic acid probe assay

A new DNA sample preparation microfluidic chip for Nucleic Acid (NA) probe assay has been proposed. The proposed microfluidic chip is composed of three parts: microfilter, micromixer and DNA purification chip. We have fabricated a microsieve type filter with an array of 2.2 /spl mu/m diameter holes. We have also demonstrated the mixing can be successfully achieved for low Reynolds number below 50 by using the fabricated micromixer. The fabricated DNA purification chip has shown a binding capacity of 15 ng/cm/sup 2/ and a minimum extractable input concentration of 100 copies/200 /spl mu/L. The proposed microfluidic chip can be applied for low-cost, disposable sample preparation of NA probe assays.