Protein addressing in packaged multi-channel by electro-click chemistry for calorimetry biosensor

Protein addressing in packaged multi-channels is developed for calorimetric biosensors in this research. First, we use electrodeposition method to fabricate chitosan film as the substrate on the patterned electrode surface. Second, the fabricated chitosan film was functionalized with alkyne groups for click chemistry. Thirdly, the azide-tagged protein is immobilized on the electrode by electrical signal for enzyme immobilization. The results demonstrate that the electro-click chemistry method was capable of multi-enzyme immobilization in packaged multi-channel for calorimetry biosensor of healthcare application.