3D multi-functional neural probe array for mapping functional connectivities in a 3D neuron chip

We present the first 3D multi-functional neural probe array integrated with optical, chemical, and electrical stimulation capabilities, developed for mapping the 3D functional connectivities among neurons cultured in a 3D hydrogel neuron chip. The proposed device is not only capable of stimulating neurons with various modalities but also tracking the propagated neural activities in the whole 3D hydrogel chip using the 3D electrode array. Using our 3D multi-functional neural probe array, we demonstrated successful in vitro experiments by electrically and chemically modulating neurons and recording neural signals on cultured neurons in the 3D hydrogel neuron chip.