Correlation of microwave power dependence and temperature dependence on enzymatic reaction under microwave irradiation

Chemistry under microwave irradiation shows specific phenomena to overturn common sense. On the other hand, these phenomena also have resulted in time shortening effect for a number of chemical processes. These effects have been led to the breakthrough innovation. Most of their specific phenomena are chemical reactions. However, the mechanisms at the molecular level, there is still a lot of discussion. As research aimed at elucidating the mechanism, we have been working on organic reactions, enzymatic reactions, and microbial cultivation. In this study, we proceeded to study about the enzymatic hydrolysis reaction of invertase and lipase as a model reaction. Invertase, keeping the reaction temperature constant, was experimentally giving various microwave power. Lipase, for reaction substrates of different polarity, examined the effect of microwave power. In order to precisely control the temperature and the microwave output, the reaction vessel was fitted with a cooling jacket. For the obtained results, physical-chemical analysis such as the Michaelis-Menten equation and the Arrhenius equation has been carried out. First, the results of invertase, the phenomenon of “microwave output dependent” was found. It is speculated that corresponds to the “temperature dependence” of an enzyme reaction. This phenomenon has been found already also in the microbial cultivation. Next, the results of lipase, correlation between the substrates of a polar and enzyme kinetics was observed. Molecular motion is controlled in rotational motion by the electromagnetic energy, it is estimated to the formation of the specific heating mechanism.