Wide-field heterodyne vibrometry for measurement of surface vibrations in biological tissues

Nanovibrations in bio-tissues play pivotal roles in organ functions, but techniques for their measurement have been premature. To address this, a wide-field vibrometry is adopted in a laser microscope. 2D surface vibration distributions of paw tissues of rats could be measured without x−y mechanical beam scanning. The measurable vibration frequency and amplitude were 10 kHz and approximately 240 nm.