Conductimetric transducer array for the readout of low-density protein microarrays

An electrical readout system of protein microarrays comprising arrays of gold interdigitated electrode pairs, which enabled impedance multiplexed detection of up to 36 biological events on the same substrate, and of polydimethylsiloxane (PDMS) microwells, is reported. Similarly to fluorescent readout counterparts, regular glass slides were applied for carrying out the microarray. However, unlike them, the presented system is compact and requires a simple and inexpensive instrumentation, thus being ideally suited for deployment of bioarray detection approaches. The system was based on the use of urease-based immunoassay formats applied to the detection of boldenone steroid achieving analytical results similar to those obtained by fluorescent scanner detection approaches.