Characterization and bioanalytical application of innovative plasmonic nanostructures

To combine fluorescence and surface enhanced Raman spectroscopy (SERS) utilizing the same plasmonic array, a silver deposited micro fabricated quartz grating prepared by electron beam lithography is used. The most efficient Raman signal amplification is achieved for molecules directly adsorbed on the metallic surface, while fluorescence is quenched under these conditions. By careful adjustment, fluorescence is also enhanced which is known as metal enhanced fluorescence. In this contribution, the combined fluorescence and SERS readout is verified by a DNA detection scheme based on the usage of the label molecule ATTO565.