A novel hypothesis-testing technique for resolving overlapping peaks in DNA sequencing

The accuracy of determining the Deoxyribo Nucleic Acid (DNA) sequence is limited by overlapping peaks of the same base-type (A, C, G or T). Commonly used iterative method to resolve these peaks tends to generate false peaks and has limited resolution. This paper presents a novel hypothesis-testing method, which uses a statistical model of DNA time series, to estimate position and amplitude of the overlapping peaks. The new method has better resolution than the iterative method and does not generate false peaks.