mRNA expression of extracellular matrix proteins following exposure of bladder smooth muscle cells to sustained hydrostatic pressure

The bladder responds to, functions under, and is controlled by mechanical forces. In vivo, pathological conditions involving elevated pressures within the bladder have been associated with increased amounts of extracellular matrix production and decreased bladder tissue compliance. The present in vitro cellular model was designed to simulate conditions pertinent to bladder function at the cellular/molecular level and examined the responses of bladder smooth muscle cells to sustained hydrostatic pressure. Specifically, the transcription of extracellular matrix proteins (such as collagen and laminin) were monitored following exposure of bladder smooth muscle cells to sustained hydrostatic pressure.