Redox cycling-based electrochemical-enzymatic biosensor platform fabricated via electrodeposition of gold nanoparticles on carbon IDA nanoelectrodes

We developed a highly sensitive biosensor platform based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on carbon interdigitated array (IDA) nanoelectrodes decorated with gold nanoparticles (AuNPs). The sensor platform fabricated using a simple, cost-effective, reproducible, and batch microfabrication process known as carbon-MEMS and electrodeposition. The 3D AuNP/carbon IDA facilitates the enhancement of surface reactivity, surface area, and reduces the inter-electrode gap; these enhance the redox-cycling-based electrochemical signal amplification. AuNP/carbon IDA is further applied to the amperometric detection of glucose and exhibited higher sensitivity and better LOD attributed its potential applicability to clinical applications.