Development of a system for measuring and controlling environmental parameters in an chamber for in vitro cell cultures experimentation

Cell cultures are essential in research. They need to be under appropriate conditions of temperature, humidity, and carbon dioxide to promote their growth. A system for monitoring and controlling the CO
₂
concentration, as well as for measuring and recording both temperature and humidity parameters inside of a conditioned chamber for experimentation with magnetic fields and recording the Transepithelial Electrical Impedance of cells, has been developed in this work. This system was designed to support the development of healthy growth of monolayer cultures and their survival. The system was based on the device K-33 BLG for CO
₂
concentration measurements and the sensor DHT22 for monitoring temperature and relative humidity. A graphical interface was developed for the control and monitoring of CO
₂
, the exhibition of temperature and relative humidity values, the definition of set points parameter, and for alerting when thresholds are exceeded. The system is autonomous and can be adapted to several types of incubators. The validation was performed using three commercial incubators as a reference, and according to the performance results, this system can be used in experimental procedures.