Gas perfusable microfabricated membranes for high-density cell culture

We report for the first time the design, fabrication, and feasibility of a high-density cell culture system capable of a cell substrate density of 40 cm
²
/cm
³
, greater than 250× current commercially available systems. This design provides oxygen directly from gas perfusable membranes on which the cells are grown to overcome oxygen delivery limitations in traditional culture systems. With translation in mind, the membranes are composed only of medical grade PDMS and Parylene to mitigate contaminant leaching concerns.