Optical tweezers with 3D high speed force feedback

Optical tweezers are one of the most versatile technique to achieve biological micromanipulation. It will benefit of automatization or teleoperation for faster achieving complex tasks. Here a new vision technique is presented with high potential for high-speed, high-resolution 3D tracking of trapped objects. The characterization of this event-based biomimetic vision is fully detailed, and the performance achieves 2kHz real-time closed loop system with a potential of improvement at 30kHz sampling rate. The working range meets fully optical tweezers requirements, and the resolution is suboptical less than 40nm in the three dimensions. By using an algorithm with shape recognition, the tracking is robust to environmental conditions of real biological samples.