Image processing for digital holograms of marine particles and organisms

A set of image processing approaches are proposed for processing digital holographic images of plankton. The images are segmented to produce regions of interest (ROI) of plankton, and then the shape description of plankton is normalized which is invariant under the transformations of translation, scaling, skewing and rotation. Also break points of the normalized shape contour curve are detected and then the curve is presented compactly by a polygon based on those points.