${\rm MS2DB}+$: A Software for Determination of Disulfide Bonds Using Multi-Ion Analysis

MS2DB+ is an open-source platform-independent web application for determining, in polynomial time, the disulfide linkages in proteins using tandem mass spectrometry (MS/MS) data. It utilizes an efficient approximation algorithm which allows the consideration of multiple ion-types (
a
,
a
_o
,
a*
,
b
,
b
_o
,
b*
,
c
,
x
,
y
,
y
_o
,
y*
, and
z
) in the analysis. Once putative disulfide bonds are identified, a graph optimization approach is used to obtain the most likely global disulfide connectivity pattern. Availability-http://haddock2.sfsu.edu/~ms2db/disulfidebond/.