Flow cytometry analysis of human interferon α/β-receptor expression on H9, raji and U937 cells

Using flow cytometry analysis with human type I interferons (IFN) (IFN-α and β and IFN-α/β-receptor entlbody (ligand binding and antibody binding), we compared hurmn IFN α/β-receptor expressions on hurmn H9 (T cells), Raji (B cells) and U937 (monocyte cells). The results indicated that H9, Paji and U937 cells all expressed IFN-α/β-receptor on their cell surfaces and the Paji cells showed the highest I evel of expression. The flow cytometry analysis using the antiserum to human IFN-α/β-receptor more clearly showed a strong IFN-α/β-receptor expression on Raj i cells and a weak expression H9 and U937 cells. These resul ts indicate that B cells (Raji) expressed rrore receptor rrolecul es on their cell surface than on T cells (r9) and on monocytes (U37), and the number of receptor molecules expressed on the cells is enough to be measured with flow cytometry.