Advanced hardware for fine-tuning and optimization of sonoporation efficiency in vitro

To our knowledge current achievements in tuning the sonoporation's bio-efficiency is limited by the inflexibility of the ultrasound excitation hardware. Advanced instrumentation for fine-tuning and optimization of sonoporation efficiency in-vitro has been developed. The advantages of developed hardware are: the fine tuning of center frequency of the burst; the fine tuning of amplitude of the burs from 50 V to 500 V; the programmable number of burst periods, duty cycle of series of bursts and repetition frequency, and total time of excitation. Our hardware together with wideband focused transducer has specified parameters in space, and ability to control the acoustic pressure amplitude with digital precision. Operation range is 500 kHz to 5 MHz; length of burst variable up to 100 periods; pulse repetition frequency up to 5 kHz. The linear RF power amplifier with matched output impedance was replaced by the low output impedance square wave generator. Such hardware solution allows having better output amplitude stability and digital programmability, yet at increased economy: both power consumption and cost of equipment. Instrumentation structure and operation principles are presented.