Nanoengineered uric acid biosensor based on chemiluminescence

Uric acid (UA) is a nitrogenous end product of the purine catabolism in humans. Excessive production of UA may lead to gout, hyperuricemia and kidney disorders. The research here aims at developing an indirect enzyme based UA biosensor where UA is sensed from the amount of hydrogen peroxide (H₂O₂) produced. Pentacene loaded nanoparticles encapsulated in uricase loaded alginate microspheres (nano-in-micro) particles were synthesized. All formulations were characterized using DLS and imaged using Optical, SEM, TEM, CLSM and FTIR. These nano-in-micro particles were used for H₂O₂ and UA sensing. UA sensing with nano-in-micro system showed regression constant 0.977 in range of 0-0.1mg/ml. Thus chemiluminescent nano-in-micro particles can act as diagnostic markers for detection of UA.