Quantitative fluorescent imaging of specific markers of diseased tissue

We have developed a random walk theory to relate observable fluorescent intensities on tissue surfaces to the presence of several embedded fluorescent masses. This work is an extension of analysis previously carried out for an isolated fluorescent site. Results depend on the optical properties of tissue at the excitation and emission wavelengths, as well as on the location of fluorescent targets and positions of source and detector. Measurements on tissue-like phantoms were performed to test the theory. Analysis of fluorescent signals, performed in accordance with the theory, yields accurate information about the location of the targets. Prospects of a noninvasive quantitative fluorescent imaging system, to diagnose and monitor salivary glands disease, is presented.