Migratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel

The aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2-3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2-6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines -10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines.