A high-throughput microfluidic chip for size sorting of cells

We present a microfluidic cell sorter that enables high-throughput, label-free, size separation of cells (ranging from few microns to hundred microns) in heterogeneous biological samples. The separation technique we use here involves microsieves filtration and time-sequential flow rate control (back-and-forth flow) separation. The results show that our design can achieve high separation efficiency (99%) in a high-throughput manner (100~1000cells/s).