Effect of light irradiation and immobilization of carbon nanotubes on kinetic parameters corresponding to Michaelis — Menten equation, for NADH sensors based on phenothiazine graphite modified electrodes

The presence of light irradiation and immobilization of carbon nanotubes on graphite electrodes modified with polyphenothiazine formaldehyde (PPF) and bis-phenothiazin-3-yl methane (BPhM) leads to an increasing of sensitivity for NADH detection (calculated as I
max
/K
M
ratio). The paper presents the analogical modelling and numerical simulation for fitting the Michaelis-Menten equation to experimental data by a regression analysis. The program determines the theoretical kinetic parameters: K
M
and I
max
and they are compared with the values of the same parameters experimentally determined.