Influence of the Solution on Adhesion Layer Polymerized in Radio Frequency Pulsed Plasma for DNA Hybridization Assay

A radio frequency (RF) pulsed plasma polymerized allylamine (PPPAA) film as an adhesion layer to adsorb DNA probe and then hybridize with a complementary or a mismatch DNA target is investigated in this paper. The possible application of plasma polymerization film as an adhesion layer for DNA hybridization assay is evaluated by DNA hybridization reactions. It was found, by surface plasmon enhanced fluorescence spectroscopy (SPFS), that the DNA hybridization reactions in phosphate buffered saline (PBS) solution can be remarkably revealed by fluorescence signals, but it heavily depends on the solution conditions. The influence of the solution pH value and the ionic strength on the PPPAA film thickness and adsorption is explored in detail.