Effects of Kochia scoparia Extracts to Activities of Several Enzymes of Tetranychus viennensis

The extract of the K. scoparia had the acardial activity against the T. viennensis. Here the female adult mites were treated by the KFCE-2 extract of K. scoparia, the effective ingredient of the extract of K. scoparia. The toxicosis symptoms of T. viennensis in response to the the KFCE-2 extract of K. scoparia were observed. After treatment, the mites went through four phases: the motionless phase (0-4 h), the excited phase (4-8 h), the convulsion phase (8-16 h) and the death phase (16-24 h). The treated mites showed the typical toxicosis symptoms, for example overexcitation, body inclination, kinetic ataxy and limb twitching. Then the protein content, the protease activity, the glutathione-S-transferase (GSTs) activity, the acetylcholine esterase (AchE) activity and the monoamine oxidase activity of the mites treated with the KFCE-2 extract of Kochia Scoparia were assayed. The results showed that the protein content and the activity of protease in T. viennensis treated with the extracts of K. Scoparia were increased and the activity of protease had the negative relationship with the protein content. The activity of GSTs was induced by the KFCE-2 extract of K. Scoparia. The activities of the AchE and the monoamine oxidase of the treated mites were inhibited. The inhibiting effects of AchE and monoamine oxidase indicated that the nervus centralis system was the main target of the extract of K. scoparia. The long time inhibition of the acetylcholinesterase activity by the KFCE-2 extract of K. scoparia blocked the nerve conduction of the treated mites and caused the treated mites to die.