Development of a Simultaneously Time- and Spectrum-Resolved Multifocal Multiphoton Microscopy System Using a Streak Camera

We report the development of a multifocal multiphoton microscope (MMM) system using a specially designed streak camera, which is capable of providing simultaneously time- and spectrum-resolved fluorescence microscopy. Temporal and spectral resolutions of the system have been calibrated to be 6.5-200 ps and 1-3 nm respectively. We demonstrate the applicability of the system to spectrum-resolved fluorescence and lifetime imaging with fluorescent dyes, fluorescent microspheres. Potential applications of the system include the measurement of tissue pathology, intracellular physiology etc.