Optofluidic microsorter: an integrated flow cytometry instrument for cell biology applications

The authors have built a high-throughput, fluorescence-activated microfluidic cell sorter based on an active, all-optical particle control switch. After being aligned to the center of the channel by flow focusing, cells are analyzed and then switched based on their detected fluorescence. Target cells are directed by the laser to the collection output while all other cells flow to the waste output.