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Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields | IEEE Conference Publication | IEEE Xplore

Response of Mammalian cells to non-thermal intense narrowband pulsed electric fields


Abstract:

This paper describes the biological effect of intense pulse electric field from the frequency point of view. A pulse-modulated sinusoidal wave as the A narrowband pulsed ...Show More

Abstract:

This paper describes the biological effect of intense pulse electric field from the frequency point of view. A pulse-modulated sinusoidal wave as the A narrowband pulsed electric field (nbPEF) allows us to deliver a non-thermal, intense and well-defined electric field in terms of frequency, field strength and deposition energy to biological systems. 10 μs long sinusoidal electric fields with a frequency range between 0.1 and 100 MHz and field strengths of up to 10 kV/cm were applied to HeLa or HeLaS3 cells, which were subsequently analyzed in terms of the morphology and the Ca2+ response. The field with the frequency below a few MHz immediately causes blebs in the external field direction, whereas the morphology does not change apparently in the case of the frequency more than 10 MHz. The intracellular Ca2+ concentration rapidly increased after the exposure to the low frequency field and subsequently decayed exponentially within hundreds of seconds. Inversely, for the high frequency fields, the Ca2+ concentration did not change for seconds after the pulse, but increased gradually in tens to hundreds of seconds. When the Ca2+ channel on the plasma membrane was inhibited, the delayed Ca2+ uptake was suppressed. Our experiment shows that the possibility to activate or impair function of membrane proteins physically by using nbPEF without significant defects of the plasma membrane.
Date of Conference: 19-24 March 2017
Date Added to IEEE Xplore: 18 May 2017
ISBN Information:
Conference Location: Paris, France

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