Computational ECG reconstruction and validation from high-resolution optical mapping

Here we present a series of improvements to the optical mapping electrocardiogram (OM-ECG), a reconstruction of the ECG based on transmembrane voltages measured on the surface of the heart. We compare our predicted OM-ECG with a pseudo-ECG concurrently measured with platinum leads surrounding the heart in-vitro. We find that the surface optical mapping of transmembrane voltages is sufficient for capturing important morphology in the ECG and moreover intramural propagation waves due to the specific transmembrane voltage sensitive dye used. Methods concerning OM-ECG reconstruction are detailed and discussed in comparison to the pseudo-ECG.