In vivo transfection microsystems

Transfection is currently used to insert molecules into cells. In vivo transfection is mainly performed via viral or chemical transfection. However, electrical transfection is known to be a more efficient way to insert drugs into cells without side effects. In spite of this advantage, not too many devices allow to perform electrotransfection in vivo because of their invasiveness. Here we present a new microfluidic microdevice which is small enough to be inserted into deep region with a minimum of drawbacks. Therapeutic molecules, genes or drugs can be injected into targeted tissues. High voltage electric impulsions can be applied. This device offers the advantage to be a stand alone device with a 500 /spl mu/m square section. This generic tool can be used for drug delivery, electrotransfection as well as electrostimulation.