Dynamic Cell and Microparticle Control via Optoelectronic Tweezers
Ohta, A.T.; Pei-Yu Chiou; Han, T.H.; Liao, J.C.; Bhardwaj, U.; McCabe, E.R.B.; Fuqu Yu; Ren Sun; Wu, M.C.
Microelectromechanical Systems, Journal of
Volume 16, Issue 3, June 2007 Page(s):491 - 499
Digital Object Identifier   10.1109/JMEMS.2007.896717
Summary:This paper reports on cell and microparticle manipulation using optically induced dielectrophoresis. Our novel optoelectronic tweezers (OET) device enables optically controlled trapping, transportation, and sorting via dielectrophoretic forces. By integrating a spatial light modulator and using direct imaging, arbitrary dynamic manipulation patterns are obtained. Here, we demonstrate manipulation functions, including particle collectors, single-particle traps, individually addressable single-particle arrays, light-defined particle channels, and size-based particle sorting. OET-induced particle manipulation velocities are analyzed as a function of the applied voltage, optical pattern linewidth, and single-particle trap dimensions.

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